Saturday, June 5, 2010

Detection of Nicotine and Its Metabolites in Urine

Official Publication Of The American Academy Of Health Behavior

Amruta R. Eswara, PhD
Saraswathy V. Nochur, PhD
Donald J. Mossman

ABSTRACT: A simple strip test has been developed for the detection of nicotine and its metabolites in urine. The NicCheck® I test yields a pink color on the test strip within 30 minutes, providing a semi-quantitative measure of tobacco consumption. When tested on urine from smokers and nonsmokers, the test was determined to have an overall sensitivity and specificity of 99% when compared to an EIA for cotinine determination. The test detects both nicotine and cotinine, as well as a number of other nicotine metabolites, thus providing a more complete indication of overall tobacco consumption. Based on its simplicity, accuracy, and ease of use, the test could conceivably have several applications.